10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex -- University of Minnesota TMCs (CG000527 Rev D)

Abstract

10x Genomics Chromium Single Cell Expression flex protocol for library construction.

Protocol ID# (CG) and Revision letter provided here:

10x protocol CG000527, Revision D -- Library construction of 10x fixed single cell or nuclei

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.

Steps

http://www.10xgenomics.com/products/single-cell-gene-expression-flex

http://www.10xgenomics.com/support/single-cell-gene-expression-flex

Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol

10x Chromium Fixed RNA Profiling, Multiplexed Samples, Rev D (Protocol CG000527) .pdf

Abstract

Steps

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