Analysis of clonogenic growth in vitro

Nikko Brix, Daniel Samaga, Claus Belka, Horst Zitzelsberger, Kirsten Lauber

Published: 2021-10-24 DOI: 10.1038/s41596-021-00615-0

Clonogenic Assay

In Vitro

Cellular Cooperation

Survival Analysis

Protocol Optimization

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References

The clonogenic assay measures the capacity of single cells to form colonies invitro. It is widely used to identify and quantify self-renewing mammalian cells derived from invitro cultures as well as from exvivo tissue preparations of different origins
Introduction
Materials
Procedure
Troubleshooting
Timing
- Cell model system-dependent assay optimization
- Main experiment
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Analysis of clonogenic growth in vitro

Supplementary information

Reporting Summary

Supplementary Table 1

Supplementary Table 2

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