Prepare 17 mm x 100 mm round-bottom culture tubes (e.g. VWR #60818-667) at Room temperature.

Place SOC recovery medium in a 37°C water bath.

Pre-warm selective plates at 37°C for 1h 0m 0s.

Place electroporation cuvettes (1 mm) and microcentrifuge tubes On ice.

As a positive control for transformation, dilute the control pUC19 by 1:5 to a final concentration of 10pg/μl using sterile water.

Thaw NEB Turbo Electrocompetent cells 37On ice (about 0h 10m 0s ) and mix cells by flicking gently.

Transfer 25µL (or the amount specified for the cuvettes) to a chilled microcentrifuge tube.

Add 1µL.

Carefully transfer the cell/DNA mix into a chilled cuvette without introducing bubbles and make sure that the cells deposit across the bottom of the cuvette.

Electroporate using the following conditions for BTX ECM 630 and Bio-Rad GenePulser electroporators:

 2.1 kV, 100 Ω, and 25 μF

The typical time constant is ~2.6 milliseconds.

Immediately add 975µL to the cuvette.

Gently mix up and down twice .

Transfer to the 17 mm x 100 mm round-bottom culture tube.

Shake vigorously (250rpm,0h 0m 0s) or rotate at 37°C for 1h 0m 0s.

Dilute the cells as appropriate, then spread 100µL-200µL onto a pre-warmed selective plate.

Incubate plates 8h 0m 0s to at 37°C.