Fecal Water Content Assay
Sabina Marciano, Roberta Marongiu
Published: 2023-10-13 DOI: 10.17504/protocols.io.kqdg3xx5zg25/v1
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Abstract
This test is used to check for signs of constipation
Before start
Steps
1.
Label, weight and record the weight of each Eppendorf tube
2.
Collect 2 pellets from each mouse and weigh.
3.
Dry the pellets in the oven at 60 degrees C for 24 hrs
4.
Weigh the pellets again after drying
5.
Calculate fecal water content according to the equation:
(fecal weight before drying − fecal weight after drying)/feces weight before drying × 100.
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