GI Transit Assay
Sabina Marciano, Roberta Marongiu
Published: 2023-10-13 DOI: 10.17504/protocols.io.eq2lyjj3mlx9/v1
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Abstract
This test is used to check for possible signs of constipation and alteration in the gut transit
Steps
1.
Fast mice for 6 hours
2.
Administer 0.3 mL Carmine red per mouse via oral gavage
3.
Monitor the mice every 15 minutes for up to 5 hours for the appearance of the first red fecal pellet
4.
The time from gavage to passage of first red pellet is recorded as total intestinal transit time.
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