RNAlater preparation
Tsu-Chun Hung
Published: 2023-07-25 DOI: 10.17504/protocols.io.eq2lyjn6wlx9/v1
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Abstract
RNAlater preparation
Steps
DEPC-treated water prepartion
1.
Prepare 0.1% DEPC-treated water and autocleave it
| A | B |
|---|---|
| Component | Volume |
| DEPC | 0.5 mL |
| DDW | 500 mL |
RNAlater preparation
2.
Add these component in 1L bottle in order
| A | B |
|---|---|
| Component | Volume |
| Ammonium sulfate | 350 g |
| DEPC-treated water | 467.5 mL |
| 1M tri-sodium citrate | 12.5 mL |
| 0.5M EDTA | 20 mL |
3. Remember to loose the lid when heating
Put the bottle on the stirring hot plate, stir till the mixture clear
Note
4.
After cool down, add 8.96mL 1M sulfuric acid to adjust pH to 5.2
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