• evo视讯(中国)

    TS Spurrs - unstained FFPE on Thermanox coverslips (TM - 013)

    sandra.crameri

    Published: 2023-05-09 DOI: 10.17504/protocols.io.3byl4b7rjvo5/v4
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    Abstract

    Processing unstained FFPE sections on Thermanox coverslips to Spurrs resin block for EM.

    Steps

    1.

    SAN:

    SPEC No:

    OPERATOR & STEPS:

    OPERATOR & STEPS:

    Dewax FFPE sections

    2.

    or X3B 0h 5m 0s

    3.

    or X3B 0h 5m 0s

    4.

    or X3B 0h 5m 0s

    Rehydrate

    5.

    100% volume 0h 5m 0s

    6.

    95% volume 0h 5m 0s

    7.

    70% volume 0h 5m 0s

    Conventional - with reduced times for 5um thick FFPE section

    8.

    2.5%% volume in 0.1Molarity (M) Sorenson's phosphate buffer 07.2 300 mosmol/kg0h 20m 0s.

    9.

    Wash buffer 0h 5m 0s

    10.

    1Mass / % volume in buffer0h 30m 0s

    11.

    70% volume 0h 10m 0s

    12.

    95% volume 0h 5m 0s

    13.

    100% volume 0h 5m 0s

    14.

    100% volume 0h 5m 0s

    15.

    100% volume Spurrs:100% volume in 1:1 ratio0h 30m 0s

    16.

    100% volume Spurrs0h 30m 0s

    17.

    100% volume Spurrs0h 30m 0s

    18.

    Oven polymerise 65°C

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