Tutorial: methods for three-dimensional visualization of archival tissue material

Tariq Sami Haddad, Peter Friedl, Navid Farahani, Darren Treanor, Inti Zlobec, Iris Nagtegaal

Published: 2021-10-28 DOI: 10.1038/s41596-021-00611-4

sample preparation

three-dimensional visualization

archival tissue

microscopy techniques

disease mechanisms

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Analysis of three-dimensional patient specimens is gaining increasing relevance for understanding the principles of tissue structure as well as the biology and mechanisms underlying disease. New technologies are improving our ability to visualize large vo
Introduction
Serial methods for tissue processing
- 3D histology
- Knife-edge scanning microscopy
Nonserial methods for tissue processing
Data processing and analysis
Selection criteria
Concluding remarks
- References
References
ACKNOWLEDGMENTS

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Tutorial: methods for three-dimensional visualization of archival tissue material

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