Yale Murine TMC - H&E staining following Phenocycler-Fusion Imaging
Archibald Enninful, Rong Fan, Jungmin Nam, Yale Pathology Tissue Services
Published: 2024-06-28 DOI: 10.17504/protocols.io.ewov19zjklr2/v1
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Abstract
Flow cell removal after Phenocycler-Fusion imaging and following H&E staining steps from Yale Pathology Tissue Services (YPTS).
Steps
Removing flow cell following CODEX imaging
1.
Remove slides from storage buffer.
2.
Immerse slides to xylene for 10 mins.
3.
Separate flow cell from the slide using a razorblades.
4.
Rinse slide with 1x PBS.
5.
Store in storage buffer until H&E staining.
Hematoxylin and eosin staining
6.
Stain with hematoxylin for 5 min.
7.
Rinse with water.
8.
Clarifier for 30 seconds.
9.
Rinse with water.
10.
Bluing for 1 min.
11.
Rinse with water.
12.
Stain with eosin 5 min.
13.
Immerse slides in graded ethanol to xylene.
14.
Add coverslip with resin mounting medium.
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