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换一换TNF ELISA Protocol
Caitlyn Henry
The purpose of this experiment is to investigate the effects of forskolin-mediated cAMP activation on TNF-ɑ secretion by LPS-treated Schwann cells. The immortalized rat RT4-D6P2T cell line (ATCC #CRL-2768) was cultured and received one of the following treatments: 0.1, 1, or 10 μg/mL of LPS, in N2 media (control) or N2 media supplemented with 2 μM of forskolin, for 3 hours. Cell media samples were collected, and the Rat TNF-alpha ELISA kit (RayBiotech, Cat #ELR-TNFa-1, Norcross, GA) was performed to quantify changes in TNF-ɑ secretion in response to the different treatment combinations.
AI 解读Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence.
Erin Bogacki, Patrick Lewis, sherbst
This protocol describes the evaluation of cellular processing of GPNMB mutants by Western Blotting and Immunofluorescent imaging in a HEK293 overexpression model.
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