Efficient generation of functional neurons from mouse embryonic stem cells via neurogenin-2 expression

Yingfei Liu, Jinzhao Wang, Thomas C. Südhof, Marius Wernig

Published: 2023-08-17 DOI: 10.1038/s41596-023-00863-2

neurogenin-2

mouse embryonic stem cells

functional neurons

synaptic networks

neuronal differentiation

AI 解读

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Hanna, J. H., Saha, K. & Jaenisch, R. Pluripotency and cellular reprogramming: facts, hypotheses, unresolved issues. Cell 143, 508–525 (2010).
Okita, K. & Yamanaka, S. Induced pluripotent stem cells: opportunities and challenges. Philos. Trans. R. Soc. Lond. B Biol. Sci. 366, 2198–2207 (2011).
Blanpain, C. et al. Stem cells assessed. Nat. Rev. Mol. Cell Biol. 13, 471–476 (2012).
Marchetto, M. C. & Gage, F. H. Modeling brain disease in a dish: really? Cell Stem Cell 10, 642–645 (2012).
Vierbuchen, T. et al. Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463, 1035–1041 (2010).
Zhang, Y. et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron 78, 785–798 (2013).
Mertens, J. et al. Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer’s patients. Cell Stem Cell 28, 1533–1548.e6 (2021).
Pak, C. et al. Cross-platform validation of neurotransmitter release impairments in schizophrenia patient-derived NRXN1-mutant neurons. Proc. Natl Acad. Sci. USA 118, e2025598118 (2021).
Patzke, C. et al. Neuromodulator signaling bidirectionally controls vesicle numbers in human synapses. Cell 179, 498–513.e22 (2019).
Marro, S. G. et al. Neuroligin-4 regulates excitatory synaptic transmission in human neurons. Neuron 103, 617–626.e6 (2019).
Huang, Y. A., Zhou, B., Wernig, M. & Südhof, T. C. ApoE2, ApoE3, and ApoE4 differentially stimulate APP transcription and Aβ secretion. Cell 168, 427–441.e1 (2017).
Yi, F. et al. Autism-associated SHANK3 haploinsufficiency causes Ih channelopathy in human neurons. Science 352, aaf2669 (2016).
Karow, M. et al. Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program. Nat. Neurosci. 21, 932–940 (2018).
Biddy, B. A. et al. Single-cell mapping of lineage and identity in direct reprogramming. Nature 564, 219–224 (2018).
Lin, H. C. et al. NGN2 induces diverse neuron types from human pluripotency. Stem Cell Rep. 16, 2118–2127 (2021).
Chanda, S. et al. Generation of induced neuronal cells by the single reprogramming factor ASCL1. Stem Cell Rep. 3, 282–296 (2014).
Treutlein, B. et al. Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq. Nature 534, 391–395 (2016).
Ang, C. E. et al. The novel lncRNA lnc-NR2F1 is pro-neurogenic and mutated in human neurodevelopmental disorders. eLife 8, e41770 (2019).
An, D. et al. Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons. eLife 8, e44423 (2019).
Höpfl, G., Gassmann, M. & Desbaillets, I. Differentiating embryonic stem cells into embryoid bodies. Methods Mol. Biol. 254, 79–98 (2004).
Paşca, A. M. et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat. Methods 12, 671–678 (2015).
Bandeira, F., Lent, R. & Herculano-Houzel, S. Changing numbers of neuronal and non-neuronal cells underlie postnatal brain growth in the rat. Proc. Natl Acad. Sci. USA 106, 14108–14113 (2009).
Clarke, L. E. & Barres, B. A. Emerging roles of astrocytes in neural circuit development. Nat. Rev. Neurosci. 14, 311–321 (2013).
Herculano-Houzel, S. The glia/neuron ratio: how it varies uniformly across brain structures and species and what that means for brain physiology and evolution. Glia 62, 1377–1391 (2014).
Zhang, Z. et al. Optogenetic manipulation of cellular communication using engineered myosin motors. Nat. Cell Biol. 23, 198–208 (2021).
Chanda, S. et al. Direct reprogramming of human neurons identifies MARCKSL1 as a pathogenic mediator of valproic acid-induced teratogenicity. Cell Stem Cell 25, 103–119.e6 (2019).
Aydin, B. et al. Proneural factors Ascl1 and Neurog2 contribute to neuronal subtype identities by establishing distinct chromatin landscapes. Nat. Neurosci. 22, 897–908 (2019).
Iacovino, M. et al. Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells. Stem Cells 29, 1580–1588 (2011).
Czechanski, A. et al. Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains. Nat. Protoc. 9, 559–574 (2014).
Valamehr, B. et al. Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies. Proc. Natl Acad. Sci. USA 105, 14459–14464 (2008).
Wu, W. et al. Neuronal enhancers are hotspots for DNA single-strand break repair. Nature 593, 440–444 (2021).

Efficient generation of functional neurons from mouse embryonic stem cells via neurogenin-2 expression

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