Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

Hiroyuki Koike, Kentaro Iwasawa, Rie Ouchi, Mari Maezawa, Masaki Kimura, Asuka Kodaka, Shozo Nishii, Wendy L. Thompson, Takanori Takebe

Published: 2021-01-10 DOI: 10.1038/s41596-020-00441-w

organoids

pluripotent stem cells

hepato-biliary-pancreatic development

inter-organ connectivity

3D culture

AI 解读

Supplementary information

Reporting Summary

Supplementary Video 1

Animated overview of the HBPO protocol

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Supplementary information

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Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

Supplementary information

Reporting Summary

Supplementary Video 1

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